| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Slayton, Alexandra A (16)
|
VACGBD |
23.91 |
24.56 |
0.65 |
| 2 |
Katzer, Danika J (16)
|
VACGBD |
23.94 |
24.82 |
0.88 |
| 3 |
Morello, Madeline J (17)
|
VACGBD |
23.64 |
26.11 |
2.47 |
| 4 |
Tinneny, Jacqueline M (16)
|
VAUN-C |
25.34 |
26.50 |
1.16 |
| 5 |
Christou, Melina C (15)
|
VACGBD |
26.11 |
26.51 |
0.40 |
| 6 |
DeWall, Skyler V (16)
|
VAUN-C |
26.30 |
26.55 |
0.25 |
| 7 |
Marstellar, Chelsea A (16)
|
VACGBD |
24.34 |
27.00 |
2.66 |
| 8 |
Clark, Casie J (17)
|
VACGBD |
26.08 |
27.08 |
1.00 |
| 9 |
Freiling, Emma C (17)
|
VACGBD |
25.07 |
27.40 |
2.33 |
| 10 |
Toledo, Hailey M (17)
|
VACGBD |
26.37 |
27.56 |
1.19 |
| 11 |
Buirski, Shea T (15)
|
VACGBD |
26.52 |
27.61 |
1.09 |
| 12 |
Coppage, Ashley G (15)
|
VACGBD |
30.10 |
28.98 |
-1.12 |
| 13 |
Gaffney, Megan N (15)
|
VACGBD |
26.40 |
29.26 |
2.86 |
| 14 |
Kreutter, Katelynn A (15)
|
VAODAC |
32.00 |
31.52 |
-0.48 |